Analysis of Immumoreactivity of Heterologously Expressed Non-structural Protein 4B (NS4B) from Hepatitis C Virus (HCV) Genotype 1a

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analysis of immumoreactivity of heterologously expressed non-structural protein 4b (ns4b) from hepatitis c virus (hcv) genotype 1a

background: detection of hepatitis c virus specific antibodies is the initial step in chronic hcv diagnosis.  hcv ns4b is among the most immunogenic hcv antigens and has been widely used in commercial enzyme immunoassays (eia). additionally, ns4b, a key protein in the virus replication, can be an alternative target for antiviral therapy.objectives: development of a new method for high-level exp...

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By a cDNA array representing 2308 signal transduction-related genes, we studied the expression profiles of HeLa cells stably transfected by Hepatitis C virus nonstructural protein 4B (HCV-NS4B). The alterations of the expression of four genes were confirmed by real-time quantitative RTPCR; and the aldo-keto reductase family 1, member C1 (AKR1C1) enzyme activity was detected in HCV-NS4B transien...

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Hepatitis C virus (HCV) envelope glycoprotein-2 (E2) inhibits the interferon (IFN)–induced, double –stranded RNA activated protein kinase (PKR) via PKR eukaryotic initiation factor-2α phosphorylation homology domain (PePHD). Present study examined the genetic variability of the PePHD in patients receiving interferon therapy. The PePHD region from HCV genotype 1a/1b infected patients receiving I...

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ژورنال

عنوان ژورنال: Iranian Journal of Biotechnology

سال: 2015

ISSN: 1728-3043,2322-2921

DOI: 10.15171/ijb.1321